Endonuclease
Description:
Endonuclease is a protein consisting of two subunits with a molecular weight of about 30 kD each. The protein has an isoelectric point (pI) at pH 6.85. It is functional between pH 6 and 10, and from 0℃to above 42℃. Mg2+ (1–2 mM) is required for enzyme activity.
Host Animal: E. coli
Format: Purified, Liquid
Buffer: 50% glycerol
Applications:Endonuclease acts as an endonuclease that degrades both DNA and RNA—whether single-stranded, double-stranded, linear, circular or supercoiled. No base preference is observed. As with all endonucleases, Endonuclease hydrolyzes internal phosphodiester bonds present between the nucleotides. Upon complete digestion, all free nucleic acids present in solution are reduced to 5’-monophosphate-terminated oligonucleotides, which are three to five bases in length.
Storage: Store at -20°C. Avoid multiple freeze/thaw cycles.